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 تاريخ مطلب : دوشنبه02/02/92

مقاله "Determination of gabapentin in human plasma using simultaneous cloud point ... by HPLC"


مقاله "Determination of gabapentin in human plasma using simultaneous cloud point extraction and precolumn derivatization by HPLC"

Zeinab Pourghobadi • Rouhollah Heydari • Roghiyeh Pourghobadi • Marzieh Rashidipour

  
 2011 Journal :  Journal of Jahrom University of Medical Sciences
Abstract 
A simultaneous cloud point extraction and precol- umn derivatization method was developed for the determination of gabapentin in human plasma samples. o-Phthaldialdehyde in the presence of 3-mercaptopropionic acid and Triton X-100 were used as derivatization reagents and extraction solvent, respectively. Some parameters with an effect on the extraction, such as the concentration of Triton X-100, incubation time, equilibration temperature, ionic strength, and centrifuging time, were studied and optimized. Under the optimum conditions, average recovery of gabapentin was in the range of 94.5–98.7 %. The calibration curve was linear in the range of 0.01–50 lg/cm3. The limits of detection and quantification were 0.003 and 0.01 lg/cm3, respectively. The relative standard deviations were in the range of 0.9–5.2 %. The obtained results show that simultaneous derivatization and cloud point extraction combined with high-performance liquid chromatography is a sensitive and simple method for the determination of gabapentin in plasma samples..
Keywords: Gabapentin , Derivatization , o-Phthaldialdehyde , Cloud point extraction , HPLC , Plasma  
 
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